不同致病性H9N2亚型禽流感病毒诱导鸡TLR-7和Mx基因mRNA转录水平的差异

doi:10.11843/j.issn.0366-6964.2017.05.015

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (5): 907-913.doi: 10.11843/j.issn.0366-6964.2017.05.015

不同致病性H9N2亚型禽流感病毒诱导鸡TLR-7和Mx基因mRNA转录水平的差异

王建琳, 曹志伟, 王冬冬, 尹燕博^*

青岛农业大学动物科技学院, 青岛 266109

收稿日期:2016-11-04 出版日期:2017-05-23 发布日期:2017-05-23
通讯作者: 尹燕博,E-mail:yanboyin2011@163.com
作者简介:王建琳(1976-),女,山西沁源人,博士,主要从事禽类传染病致病机制及防控研究,Tel:0532-86080734,E-mail:bjsxxw@126.com;曹志伟为同等贡献作者
基金资助:
国家自然科学基金项目（31272535）；山东省现代农业产业技术体系项目（SDAIT-11-03）；山东省自然科学基金（ZR2013CL022）

Transcriptional Responses of TLR-7 and Mx mRNA in 3 Chicken Host Systems Infected with H9N2 Influenza Viruses with Different Pathogenicities

WANG Jian-lin, CAO Zhi-wei, WANG Dong-dong, YIN Yan-bo^*

College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China

Received:2016-11-04 Online:2017-05-23 Published:2017-05-23

摘要/Abstract

摘要：

本试验拟研究不同致病性H9N2亚型禽流感病毒诱导SPF鸡、SPF鸡胚和鸡胚成纤维细胞中TLR-7和Mx基因mRNA转录水平的动态变化，为不同致病性H9N2亚型禽流感病毒诱导IFN-Ⅰ产生差异的机制提供依据。150只6周龄SPF鸡随机分为3组，分别静脉接种PBS、10⁵EID₅₀毒株SD/196和SD/818各0.2 mL，分别于接种后的第1、3、5、7和10天每组剖杀8只，收集具有明显病变差异的组织；180枚10日龄SPF鸡胚分别尿囊腔接种PBS、10⁴EID₅₀的SD/196毒株和SD/818毒株各0.1 mL，分别于接种后的第4、8、16、24、32和48小时每组收集胚体10个；鸡胚成纤维细胞分别接种PBS、SD/196和SD/818毒株病毒液（1 moi）进行37℃培养，于感染后的第4、8、16、24、32和48小时收集细胞；动态收集的病变组织、胚体和成纤维细胞通过荧光定量PCR测定TLR-7和Mx基因mRNA水平的表达。结果显示，肺、肾、十二指肠和法氏囊为主要病变差异组织，除鸡胚TLR-7的表达外，毒株SD/818大部分时间点诱导主要病变差异组织、鸡胚和成纤维细胞中TLR-7和Mx基因mRNA的表达显著高于毒株SD/196（P<0.05）。结果表明不同致病性H9N2亚型禽流感病毒明显诱导了SPF鸡主要病变差异组织、SPF鸡胚和鸡胚成纤维细胞中TLR-7和Mx基因mRNA的表达，且较高致病性毒株SD/818诱导表达量明显高于较低致病性毒株SD/196。

Abstract:

For a better understanding of the mechanism underlying the expression of type Ⅰ IFN induced by H9N2 influenza viruses with different pathogenicity, mRNA of TLR-7 and Mx were dynamically studied in 3 host systems of chicken which infected with strains SD/818 and SD/196 in this study. SPF chickens, SPF chicken embryos and primary chicken embryo fibroblasts were randomly divided into three groups, control group, SD/196-infected group and SD/818-infected group. The lungs, kidneys, duodenum, bursa of Fabricius, embryonic bodies and primary chicken embryo fibroblasts were collected at different time points after infection, and mRNA of TLR-7 and Mx were dynamically studied using real-time quantitative reverse transcription PCR. The results showed that transcriptional level of TLR-7 and Mx in chicken host systems induced by SD/818 were significantly higher than those induced by SD/196 except expression of TLR-7 at chicken embryos. The results suggested that expression of TLR-7 and Mx mRNA in chicken host systems were induced by H9N2 influenza virus with different pathogenicity, and transcriptional level of TLR-7 and Mx induced by SD/818 were higher than those induced by SD/196.

中图分类号:

S852.659.5

王建琳, 曹志伟, 王冬冬, 尹燕博. 不同致病性H9N2亚型禽流感病毒诱导鸡TLR-7和Mx基因mRNA转录水平的差异[J]. 畜牧兽医学报, 2017, 48(5): 907-913.

WANG Jian-lin, CAO Zhi-wei, WANG Dong-dong, YIN Yan-bo. Transcriptional Responses of TLR-7 and Mx mRNA in 3 Chicken Host Systems Infected with H9N2 Influenza Viruses with Different Pathogenicities[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 907-913.

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不同致病性H9N2亚型禽流感病毒诱导鸡TLR-7和Mx基因mRNA转录水平的差异

Transcriptional Responses of TLR-7 and Mx mRNA in 3 Chicken Host Systems Infected with H9N2 Influenza Viruses with Different Pathogenicities

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